What is Bacteriostatic Water?
Bacteriostatic Water (BW) is sterile, non-pyrogenic water for injection that contains a small amount of benzyl alcohol (typically 0.9%) as a preservative. It is widely used in peptide and protein research for reconstituting lyophilized compounds into solution, especially when multiple withdrawals from the same vial are required. The presence of benzyl alcohol inhibits microbial growth, extending the usability of reconstituted peptides under proper laboratory conditions.
Composition
- Water: Sterile Water for Injection, USP
- Preservative: Benzyl Alcohol 0.9% (v/v)
- pH Range: ~4.5–7.0 (depending on the manufacturer)
- Osmolarity: Hypotonic (not buffered)
Benefits for In Vitro Research
- Microbial Growth Inhibition: The 0.9% benzyl alcohol content acts as a bacteriostatic agent, allowing repeated use from multi-dose vials within a short time frame under aseptic conditions.
- Convenient Reconstitution Medium: Easily dissolves most peptides and proteins intended for in vitro research applications.
- Improved Shelf Stability of Reconstituted Peptides: Supports short-term refrigerated storage of peptide solutions without promoting bacterial contamination.
- Sterile and Non-Pyrogenic: Suitable for sensitive biological assays, including those involving cell cultures and enzyme kinetics, when properly diluted.
Applications in In Vitro Research
- Peptide and protein reconstitution for receptor-binding, metabolic, and enzymatic studies.
- Short-term storage of peptide solutions, especially where repeated aliquots are required.
- Preparation of standards and diluents for high-precision analytical assays (e.g., HPLC, mass spectrometry).
- Formulation of test solutions for dose-response or time-course studies using peptide-based agents.
Guidelines for Use
- Use sterile techniques when withdrawing and reconstituting peptides.
- Store reconstituted peptides at 2–8 °C and use within the recommended timeframe (typically 14–28 days).
- Do not use Bacteriostatic Water for assays involving benzyl alcohol-sensitive enzymes or organisms without proper controls.
- Avoid combining with peptides that are unstable in aqueous media or require acidic reconstitution solutions.
Specifications
| Feature | Detail |
| Product Name | Bacteriostatic Water |
| Preservative | Benzyl Alcohol 0.9% (v/v) |
| Water Quality | Sterile, pyrogen-free, meets USP Water for Injection standards |
| pH Range | 4.5 – 7.0 |
| Use Case | Peptide/protein reconstitution for in vitro research |
| Packaging | Multi-dose vials (e.g., 10 mL, 30 mL) in sterile format |
| Storage | Room temperature before opening; refrigerate after use |
| Shelf Life (opened) | Typically 28 days refrigerated post-initial use |
Disclaimer
This product is intended for research purposes only. It is not approved for human or veterinary use, nor is it intended for clinical, diagnostic, or therapeutic applications. All research must comply with applicable local laws and institutional guidelines. Misuse of this compound for human consumption is strictly prohibited.
