PAN-AMK Activator O304 50mg Tablet x 60

A Comprehensive Overview for Biological and Medical Researchers

Introduction

O-304 is a novel, orally bioavailable small molecule that functions as a pan-activator of AMP-activated protein kinase (AMPK). AMPK is a central regulator of cellular energy homeostasis, and its activation by O-304 has been shown to influence various metabolic pathways. This makes O-304 a promising candidate for research into metabolic disorders such as type 2 diabetes and associated cardiovascular complications.

Chemical Structure and Properties

• Chemical Name: O-304
• CAS Number: 1261289-04-6
• Molecular Formula: C₂₀H₂₄N₂O₄
• Molecular Weight: 356.42 g/mol
• Chemical Structure: O-304 is a small molecule characterized by specific functional groups that facilitate its interaction with the AMPK enzyme, enhancing its activation.(pmc.ncbi.nlm.nih.gov, en.wikipedia.org)

Mechanism of Action

O-304 enhances AMPK activity by preventing the dephosphorylation of the Thr172 residue on the α-subunit of AMPK, a critical step for its activation. This action is dependent on the presence of the upstream kinase LKB1, as demonstrated in studies where O-304 failed to activate AMPK in LKB1-deficient HeLa cells. Unlike some other AMPK activators, O-304 does not rely on altering cellular ATP levels, thereby offering a distinct mechanism of action.

Research Applications

In preclinical studies, O-304 has shown several beneficial effects:

• Improved Glucose Homeostasis: O-304 administration led to reduced fasting plasma glucose levels and improved insulin sensitivity in animal models.(caymanchem.com)
• Enhanced Microvascular Perfusion: The compound improved peripheral microvascular perfusion, which is often compromised in metabolic disorders.
• Cardiovascular Benefits: O-304 treatment resulted in lowered blood pressure and improved cardiac function in aged mice, suggesting potential benefits for age-related cardiovascular issues.

These findings indicate that O-304 may be a valuable tool for researchers investigating metabolic and cardiovascular diseases.

Future Research Directions

Given its unique mechanism and observed benefits in preclinical models, O-304 presents several avenues for future research:

• Metabolic Disorders: Further studies could explore O-304’s potential in treating various metabolic conditions beyond type 2 diabetes, such as obesity and metabolic syndrome.
• Cardiovascular Health: Research into O-304’s effects on cardiovascular function could provide insights into its utility in preventing or treating heart diseases.
• Aging and Longevity: Considering its benefits in aged animal models, O-304 may be investigated for its role in aging and lifespan extension.

References:

1. Steneberg, P., et al. (2018). PAN-AMPK activator O304 improves glucose homeostasis and microvascular perfusion in mice and type 2 diabetes patients. JCI Insight, 3(12), e99114. Link
2. MedChemExpress. O-304 | AMPK Activator. Link
3. Selleck Chemicals. O-304 (O-304X) | AMPK activator. Link

Disclaimer

This product is intended for research purposes only. It is not approved for human or veterinary use, nor is it intended for clinical, diagnostic, or therapeutic applications. All research must comply with applicable local laws and institutional guidelines. Misuse of this compound for human consumption is strictly prohibited.