SLU-PP-332 10mg

SLU-PP-332: A Comprehensive Overview for Biology and Medical Researchers 

What is SLU‑PP‑332? 

SLU‑PP‑332 (CAS 303760‑60‑3) is a small-molecule pan‑estrogen‑related receptor (ERR) agonist, particularly selective for ERRα with an EC₅₀ of 98 nM, and also active on ERRβ (230 nM) and ERRγ (430 nM) (Tocris Bioscience). As a hydrazone derivative of 4-hydroxybenzamide and 2-naphthaldehyde, SLU‑PP‑332 modulates nuclear receptor activity with high specificity. 

Chemical Structure & Properties 

• Chemical Name: (E)-4-Hydroxy-N′-(naphthalen-2-ylmethylene)benzohydrazide 

• Molecular Formula: C₁₈H₁₄N₂O₂ 

• Molecular Weight: 290.32 Da (Wikipedia, Tocris Bioscience, ChemScene) 

• Structural Features: A hydrazone linkage between a phenolic group and naphthalene moiety, with the active Z-configuration at the imine bond (Wikipedia). 

• Purity: Typically ≥98% by HPLC (Tocris Bioscience). 

• Solubility: Soluble in DMSO (~29 mg/mL); moderate aqueous solubility may require solvent optimization (Tocris Bioscience). 

SLU‑PP‑332 is a non-peptide small molecule—not applicable for amino acid sequence or peptide modifications. 

 Mechanism of Action 

SLU‑PP‑332 activates ERRs—especially ERRα—by stabilizing their active conformation, enhancing affinity for PGC‑1α co-activators (Wikipedia). This leads to simulations of energy-demanding states, increasing: 

• Mitochondrial biogenesis via upregulation of TFAM and OXPHOS genes 

• Fatty acid oxidation, through CPT1 and MCAD enhancement 

• AMPK pathway activation, promoting metabolic adaptation 

These effects mimic physiological responses to exercise and caloric stress (Wikipedia, UF News). 

 Research-Proven Benefits 

In vitro studies demonstrate: 

• Enhanced mitochondrial respiration in muscle cells (e.g., C2C12 myocytes) (Amazon). 

• Upregulation of ERR target genes such as Pdk4 (Amazon). 

In vivo (preclinical) findings show: 

• Reduced fat mass, improved glucose tolerance, and insulin sensitivity in metabolic syndrome models (Tocris Bioscience). 

• Increased endurance, oxidative muscle fiber composition, and running capacity (~30–40%) . 

• Mitochondrial restoration and reduced inflammation in aged kidneys and hearts via ERR agonism (AdipoGen). 

Research Applications for In Vitro Studies 

SLU‑PP‑332 is ideal for exploring: 

• Mitochondrial function assays, e.g., OCR/ECAR measurements 

• Metabolic gene expression, especially PGC-1α, TFAM, CPT1 

• Signal pathway analysis, including AMPK, via western blotting or reporter assays 

• Outcomes in muscle/myotube and hepatocyte models related to fatty acid and glucose metabolism 

• Mechanistic screening of mitochondrial activators and metabolic modulators 

Future Research Directions 

• Organoid or co-culture systems to study ERR-driven metabolic crosstalk 

• Comparative pharmacology vs selective GSK4716 or XCT790 to probe receptor subtype activity 

• Proteomics and transcriptomics following compound exposure in human cells 

• Dermal cell models exploring mitochondrial or metabolic skin rejuvenation effects 

• Neuroprotective or cardiac models to evaluate ERR-mediated mitochondrial benefits 

References 

1. Tocris/Bio-Techne datasheet: SLU‑PP‑332 as pan‑ERR agonist (ERRα EC₅₀=98 nM; ERRβ=230 nM; ERRγ=430 nM); mitochondrial and in vivo metabolic effects (Tocris Bioscience, Wikipedia, AdipoGen, Focus Biomolecules, Peptide Sciences). 

1. ACS Chem. Biol. (Billon, 2023): SLU‑PP‑332 induces exercise-like metabolic response via ERRα activation (Tocris Bioscience). 

1. J. Pharmacol. Exp. Ther. (Billon, 2024): alleviates metabolic syndrome via mitochondrial mechanisms (AdipoGen). 

1. Nature news report: Exercise-mimetic benefits, energy expenditure increase (UF News). 

1. Peptide Sciences summary: activation of ERR targets and mitochondrial enhancement in vitro (Amazon). 

 Disclaimer 

This product is intended for research purposes only. It is not approved for human or veterinary use, nor is it intended for clinical, diagnostic, or therapeutic applications. All research must comply with applicable local laws and institutional guidelines. Misuse of this compound for human consumption is strictly prohibited.